Department of Anesthesiology

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PGI2 synthesis and excretion in dog kidney: evidence for renal PG compartmentalization.

TitlePGI2 synthesis and excretion in dog kidney: evidence for renal PG compartmentalization.
Publication TypeJournal Article
Year of Publication1986
AuthorsBoyd RM, Nasjletti A, Heerdt PM, Baer PG
JournalAm J Physiol
Volume250
Issue1 Pt 2
PaginationF58-65
Date Published1986 Jan
ISSN0002-9513
Keywords6-Ketoprostaglandin F1 alpha, Animals, Arachidonic Acid, Arachidonic Acids, Body Fluid Compartments, Body Fluids, Bradykinin, Dinoprostone, Dogs, Epoprostenol, Female, Injections, Intra-Arterial, Inulin, Kidney, Male, Prostaglandins E, Renal Artery, Renal Veins, Tritium
Abstract

To assess the concept of compartmentalization of renal prostaglandins (PG), we compared entry of PGE2 and the PGI2 metabolite 6-keto-PGF1 alpha into the renal vascular and tubular compartments, in sodium pentobarbital-anesthetized dogs. Renal arterial 6-keto-PGF1 alpha infusion increased both renal venous and urinary 6-keto-PGF1 alpha outflow. In contrast, renal arterial infusion of arachidonic acid (AA) or bradykinin (BK) increased renal venous 6-keto-PGF1 alpha outflow but had no effect on its urinary outflow. Both urinary and renal venous PGE2 outflows increased during AA or BK infusion. Ureteral stopped-flow studies revealed no postglomerular 6-keto-PGF1 alpha entry into tubular fluid. During renal arterial infusion of [3H]PGI2 and inulin, first-pass 3H clearance was 40% of inulin clearance; 35% of urinary 3H was 6-keto-PGF1 alpha, and two other urinary metabolites were found. During renal arterial infusion of [3H]6-keto-PGF1 alpha and inulin, first-pass 3H clearance was 150% of inulin clearance; 75% of urinary 3H was 6-keto-PGF1 alpha, and only one other metabolite was found. We conclude that in the dog PGE2 synthesized in the kidney enters directly into both the renal vascular and tubular compartments, but 6-keto-PGF1 alpha of renal origin enters directly into only the renal vascular compartment.

Alternate JournalAm. J. Physiol.
PubMed ID3079968
Grant ListHL-00806 / HL / NHLBI NIH HHS / United States
HL-18579 / HL / NHLBI NIH HHS / United States
HL-23548 / HL / NHLBI NIH HHS / United States