Department of Anesthesiology

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In vitro proton and phosphorus NMR spectroscopic analysis of murine (C57Bl/6J) brain development.

TitleIn vitro proton and phosphorus NMR spectroscopic analysis of murine (C57Bl/6J) brain development.
Publication TypeJournal Article
Year of Publication1999
AuthorsYao FS, Caserta MT, Wyrwicz AM
JournalNMR Biomed
Volume12
Issue7
Pagination463-70
Date Published1999 Nov
ISSN0952-3480
KeywordsAging, Animals, Aspartic Acid, Brain, Brain Chemistry, Chloroform, Creatine, gamma-Aminobutyric Acid, Glutamic Acid, Magnetic Resonance Spectroscopy, Methanol, Mice, Mice, Inbred C57BL, Phosphatidylcholines, Phosphatidylinositols, Phosphorus, Protons, Taurine
Abstract

We report for the first time in vitro proton and phosphorus NMR spectroscopic analyses of murine brain development from fetal to adult stages. Chloroform-methanol extracts from C57B16/J mouse brain, at ages ranging from 15 days in utero (F15) to adult, permitted the simultaneous investigation of both cytosolic and membrane phospholipid compartments. The protein content of murine brain was determined and used for quantitation of individual metabolite levels. Proton NMR spectroscopy revealed that NAA, considered a neuronal marker, is undetectable at F15. Glutamate, GABA and creatine, however, are present at this time. All four compounds reach maximum levels at 21 days postnatal (P21). Choline and alanine levels are at their peak in fetal brain and progressively fall as the brain develops. Phosphorus NMR spectroscopy shows that phosphatidylcholine, phosphatidylinositol, sphingomyelin, and phosphatidylserine increase steadily from F15 to P21.

Alternate JournalNMR Biomed
PubMed ID10654293
Grant ListNIMH R07-01056 / MH / NIMH NIH HHS / United States
R01 GM-53175 / GM / NIGMS NIH HHS / United States