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Department of Anesthesiology

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Immunoglobulin E receptor cross-linking induces oscillations in intracellular free ionized calcium in individual tumor mast cells.

TitleImmunoglobulin E receptor cross-linking induces oscillations in intracellular free ionized calcium in individual tumor mast cells.
Publication TypeJournal Article
Year of Publication1989
AuthorsMillard PJ, Ryan TA, Webb WW, Fewtrell C
JournalJ Biol Chem
Date Published1989 Nov 25
KeywordsAdenosine Triphosphate, Animals, Antigens, Differentiation, B-Lymphocyte, Benzofurans, Calcium, Cell Line, Cell Membrane, Fluorescent Dyes, Fura-2, Immunoglobulin G, Kinetics, Leukemia, Basophilic, Acute, Mast Cells, Membrane Potentials, Oscillometry, Rats, Receptors, Fc, Receptors, IgE, Sulfinpyrazone

Fura-2 fluorescence in single rat basophilic leukemia cells was monitored to study the rise in intracellular free ionized calcium ([Ca2+]i) produced by aggregation of immunoglobulin E receptors. Repetitive transient increases in [Ca2+]i were induced by antigen stimulation and were measured using digital video imaging microscopy at high time resolution. The [Ca2+]i oscillations were not dependent upon changes in the membrane potential of the cells and were observed in cells stimulated with antigen either with or without extracellular Ca2+. Transient oscillations in [Ca2+]i were also observed when calcium influx was blocked with La3+. These results suggested that during antigen stimulation of cells under normal physiological conditions, release of Ca2+ from intracellular stores makes an important contribution to the initial increase in [Ca2+]i. Oscillations in [Ca2+]i are not induced by elevating [Ca2+]i with the calcium ionophore ionomycin. Mitochondrial calcium buffering is not required for [Ca2+]i oscillations to occur. The results show that rat basophilic leukemia cells have significant stores of calcium and that release of calcium from these stores can participate in both the initial rise and the oscillations in [Ca2+]i.

Alternate JournalJ. Biol. Chem.
PubMed ID2531141
Grant List08-T1RRO4224A / RR / NCRR NIH HHS / United States
GM 33028 / GM / NIGMS NIH HHS / United States