Title | Protein phosphatase-2A is activated in pig brain following cardiac arrest and resuscitation. |
Publication Type | Journal Article |
Year of Publication | 2008 |
Authors | Zhang TT, Platholi J, Heerdt PM, Hemmings HC, Tung HYL |
Journal | Metab Brain Dis |
Volume | 23 |
Issue | 1 |
Pagination | 95-104 |
Date Published | 2008 Mar |
ISSN | 0885-7490 |
Keywords | Animals, Apoptosis, Brain, Brain Ischemia, Cardiopulmonary Resuscitation, Cell Death, Enzyme Activation, Enzyme Inhibitors, Heart Arrest, Necrosis, Protein Phosphatase 2, Reperfusion Injury, Swine |
Abstract | Protein phosphatase-2A (PP-2A) interacts with several regulators of cell death pathways and is therefore a potential component of signaling pathways linking global cerebral ischemia to cell death. Using a novel procedure to quantify PP-2A activity, we find that cardiac arrest with resuscitation and reperfusion leads to activation of PP-2A by 1.6-fold in pig brain extract and by 3.4-fold after partial purification of PP-2A. This is the first demonstration of PP-2A activation in a clinically relevant model of transient global cerebral ischemia. These results suggest that inhibition of PP-2A activity may be neuroprotective in global cerebral ischemia. |
DOI | 10.1007/s11011-007-9074-1 |
Alternate Journal | Metab Brain Dis |
PubMed ID | 18197471 |
Grant List | R01 NS056315 / NS / NINDS NIH HHS / United States |