Department of Anesthesiology

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Protein phosphatase-2A is activated in pig brain following cardiac arrest and resuscitation.

TitleProtein phosphatase-2A is activated in pig brain following cardiac arrest and resuscitation.
Publication TypeJournal Article
Year of Publication2008
AuthorsZhang TT, Platholi J, Heerdt PM, Hemmings HC, Tung HYL
JournalMetab Brain Dis
Volume23
Issue1
Pagination95-104
Date Published2008 Mar
ISSN0885-7490
KeywordsAnimals, Apoptosis, Brain, Brain Ischemia, Cardiopulmonary Resuscitation, Cell Death, Enzyme Activation, Enzyme Inhibitors, Heart Arrest, Necrosis, Protein Phosphatase 2, Reperfusion Injury, Swine
Abstract

Protein phosphatase-2A (PP-2A) interacts with several regulators of cell death pathways and is therefore a potential component of signaling pathways linking global cerebral ischemia to cell death. Using a novel procedure to quantify PP-2A activity, we find that cardiac arrest with resuscitation and reperfusion leads to activation of PP-2A by 1.6-fold in pig brain extract and by 3.4-fold after partial purification of PP-2A. This is the first demonstration of PP-2A activation in a clinically relevant model of transient global cerebral ischemia. These results suggest that inhibition of PP-2A activity may be neuroprotective in global cerebral ischemia.

DOI10.1007/s11011-007-9074-1
Alternate JournalMetab Brain Dis
PubMed ID18197471
Grant ListR01 NS056315 / NS / NINDS NIH HHS / United States