Department of Anesthesiology

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Isoflurane inhibits NaChBac, a prokaryotic voltage-gated sodium channel.

TitleIsoflurane inhibits NaChBac, a prokaryotic voltage-gated sodium channel.
Publication TypeJournal Article
Year of Publication2007
AuthorsOuyang W, Jih T-Y, Zhang T-T, Correa AM, Hemmings HC
JournalJ Pharmacol Exp Ther
Volume322
Issue3
Pagination1076-83
Date Published2007 Sep
ISSN0022-3565
KeywordsAnesthetics, Inhalation, Bacillus, Bacterial Proteins, Cell Line, Electrophysiology, Humans, Inhibitory Concentration 50, Ion Channel Gating, Isoflurane, Patch-Clamp Techniques, Sodium Channels, Transfection
Abstract

Volatile anesthetics inhibit mammalian voltage-gated Na(+) channels, an action that contributes to their presynaptic inhibition of neurotransmitter release. We measured the effects of isoflurane, a prototypical halogenated ether volatile anesthetic, on the prokaryotic voltage-gated Na(+) channel from Bacillus halodurans (NaChBac). Using whole-cell patch-clamp recording, human embryonic kidney 293 cells transfected with NaChBac displayed large inward currents (I(Na)) that activated at potentials of -60 mV or higher with a peak voltage of activation of 0 mV (from a holding potential of -80 mV) or -10 mV (from a holding potential of -100 mV). Isoflurane inhibited I(Na) in a concentration-dependent manner over a clinically relevant concentration range; inhibition was significantly more potent from a holding potential of -80 mV (IC(50) = 0.35 mM) than from -100 mV (IC(50) = 0.48 mM). Isoflurane positively shifted the voltage dependence of peak activation, and it negatively shifted the voltage dependence of end steady-state activation. The voltage dependence of inactivation was negatively shifted with no change in slope factor. Enhanced inactivation of I(Na) was 8-fold more sensitive to isoflurane than reduction of channel opening. In addition to tonic block of closed and/or open channels, isoflurane enhanced use-dependent block by delaying recovery from inactivation. These results indicate that a prokaryotic voltage-gated Na(+) channel, like mammalian voltage-gated Na(+) channels, is inhibited by clinical concentrations of isoflurane involving multiple state-dependent mechanisms. NaChBac should provide a useful model for structure-function studies of volatile anesthetic actions on voltage-gated ion channels.

DOI10.1124/jpet.107.122929
Alternate JournalJ. Pharmacol. Exp. Ther.
PubMed ID17569823
Grant ListGM58055 / GM / NIGMS NIH HHS / United States
GM68044 / GM / NIGMS NIH HHS / United States
R01 GM058055 / GM / NIGMS NIH HHS / United States