Department of Anesthesiology

You are here

Intrathecal veratridine administration increases minimum alveolar concentration in rats.

TitleIntrathecal veratridine administration increases minimum alveolar concentration in rats.
Publication TypeJournal Article
Year of Publication2008
AuthorsZhang Y, Sharma M, Eger EI, Laster MJ, Hemmings HC, R Harris A
JournalAnesth Analg
Volume107
Issue3
Pagination875-8
Date Published2008 Sep
ISSN1526-7598
KeywordsAnesthesia, Anesthetics, Animals, Dose-Response Relationship, Drug, Immobilization, Inhibitory Concentration 50, Injections, Spinal, Isoflurane, Male, Pulmonary Alveoli, Rats, Rats, Long-Evans, Sodium Channels, Subarachnoid Space, Veratridine
Abstract

BACKGROUND: Results from several studies point to sodium channels as potential mediators of the immobility produced by inhaled anesthetics. We hypothesized that the intrathecal administration of veratridine, a drug that enhances the activity or effect of sodium channels, should increase MAC.

METHODS: We measured the change in isoflurane MAC caused by intrathecal infusion of various concentrations of veratridine into the lumbothoracic subarachnoid space of rats. We compared these result with those obtained from intracerebroventricular infusion.

RESULTS: As predicted, intrathecal infusion of veratridine increased MAC. The greatest infused concentration (25 microM) also produced neuronal injury in the hindlimbs of two rats and decreased the peak effect on MAC. A concentration of 1.6 microM produced the largest (21%) increase in MAC. Intraventricular infusion of 1.6 and 6.4 microM veratridine did not alter MAC. Rats given 25 microM died.

CONCLUSIONS: Intrathecal administration of veratradine increases MAC of isoflurane, a finding consistent with a role for sodium channels as potential mediators of the immobility produced by inhaled anesthetics.

DOI10.1213/ane.0b013e3181815fbc
Alternate JournalAnesth. Analg.
PubMed ID18713899
PubMed Central IDPMC2587212
Grant List1P01GM47818 / GM / NIGMS NIH HHS / United States
P01 GM047818-14 / GM / NIGMS NIH HHS / United States
R01 GM058055 / GM / NIGMS NIH HHS / United States