|Title||Inhibitors of myosin light chain kinase block synaptic vesicle pool mobilization during action potential firing.|
|Publication Type||Journal Article|
|Year of Publication||1999|
|Date Published||1999 Feb 15|
|Keywords||Action Potentials, Animals, Azepines, Cells, Cultured, Endocytosis, Enzyme Inhibitors, Fluorescent Dyes, Kinetics, Microscopy, Confocal, Myosin-Light-Chain Kinase, Pyridinium Compounds, Quaternary Ammonium Compounds, Rats, Rats, Sprague-Dawley, Synaptic Vesicles|
During repetitive action potential firing the maintenance of synaptic transmission relies on a continued supply of synaptic vesicles for fusion with the presynaptic plasma membrane. The mechanism of transport by which vesicles are delivered to the site of fusion from a reserve pool is unknown, as are the biochemical pathways linking intracellular Ca2+ elevation with vesicle mobilization. Here, using the fluorescent tracer FM1-43 in hippocampal synaptic terminals, I show that inhibitors of myosin light chain kinase can block mobilization of the reserve pool and not the immediately releasable pool.
|Alternate Journal||J. Neurosci.|
|Grant List||NS 36942 / NS / NINDS NIH HHS / United States|