Department of Anesthesiology

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Inhibitors of myosin light chain kinase block synaptic vesicle pool mobilization during action potential firing.

TitleInhibitors of myosin light chain kinase block synaptic vesicle pool mobilization during action potential firing.
Publication TypeJournal Article
Year of Publication1999
AuthorsRyan TA
JournalJ Neurosci
Volume19
Issue4
Pagination1317-23
Date Published1999 Feb 15
ISSN0270-6474
KeywordsAction Potentials, Animals, Azepines, Cells, Cultured, Endocytosis, Enzyme Inhibitors, Fluorescent Dyes, Kinetics, Microscopy, Confocal, Myosin-Light-Chain Kinase, Pyridinium Compounds, Quaternary Ammonium Compounds, Rats, Rats, Sprague-Dawley, Synaptic Vesicles
Abstract

During repetitive action potential firing the maintenance of synaptic transmission relies on a continued supply of synaptic vesicles for fusion with the presynaptic plasma membrane. The mechanism of transport by which vesicles are delivered to the site of fusion from a reserve pool is unknown, as are the biochemical pathways linking intracellular Ca2+ elevation with vesicle mobilization. Here, using the fluorescent tracer FM1-43 in hippocampal synaptic terminals, I show that inhibitors of myosin light chain kinase can block mobilization of the reserve pool and not the immediately releasable pool.

Alternate JournalJ. Neurosci.
PubMed ID9952409
Grant ListNS 36942 / NS / NINDS NIH HHS / United States