Department of Anesthesiology

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Identifying coupled clusters of allostery participants through chemical shift perturbations.

TitleIdentifying coupled clusters of allostery participants through chemical shift perturbations.
Publication TypeJournal Article
Year of Publication2019
AuthorsXu Y, Zhang D, Rogawski R, Nimigean CM, McDermott AE
JournalProc Natl Acad Sci U S A
Volume116
Issue6
Pagination2078-2085
Date Published2019 Feb 05
ISSN1091-6490
Abstract

Allosteric couplings underlie many cellular signaling processes and provide an exciting avenue for development of new diagnostics and therapeutics. A general method for identifying important residues in allosteric mechanisms would be very useful, but remains elusive due to the complexity of long-range phenomena. Here, we introduce an NMR method to identify residues involved in allosteric coupling between two ligand-binding sites in a protein, which we call chemical shift detection of allostery participants (CAP). Networks of functional groups responding to each ligand are defined through correlated NMR perturbations. In this process, we also identify allostery participants, groups that respond to both binding events and likely play a role in the coupling between the binding sites. Such residues exhibit multiple functional states with distinct NMR chemical shifts, depending on binding status at both binding sites. Such a strategy was applied to the prototypical ion channel KcsA. We had previously shown that the potassium affinity at the extracellular selectivity filter is strongly dependent on proton binding at the intracellular pH sensor. Here, we analyzed proton and potassium binding networks and identified groups that depend on both proton and potassium binding (allostery participants). These groups are viewed as candidates for transmitting information between functional units. The vital role of one such identified amino acid was validated through site-specific mutagenesis, electrophysiology functional studies, and NMR-detected thermodynamic analysis of allosteric coupling. This strategy for identifying allostery participants is likely to have applications for many other systems.

DOI10.1073/pnas.1811168116
Alternate JournalProc. Natl. Acad. Sci. U.S.A.
PubMed ID30679272
PubMed Central IDPMC6369819
Grant ListC06 RR015495 / RR / NCRR NIH HHS / United States
P41 GM118302 / GM / NIGMS NIH HHS / United States
R01 GM088352 / GM / NIGMS NIH HHS / United States
R01 GM088724 / GM / NIGMS NIH HHS / United States