Department of Anesthesiology

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Hydrogen exchange in the formation of dihydroxyacetone phosphate from acyl dihydroxyacetone phosphate in O-alkyl lipid synthesis in Ehrlich ascites tumor cell microsomes.

TitleHydrogen exchange in the formation of dihydroxyacetone phosphate from acyl dihydroxyacetone phosphate in O-alkyl lipid synthesis in Ehrlich ascites tumor cell microsomes.
Publication TypeJournal Article
Year of Publication1981
AuthorsFriedberg SJ, Gomillion M
JournalJ Biol Chem
Volume256
Issue1
Pagination291-5
Date Published1981 Jan 10
ISSN0021-9258
KeywordsAnimals, Carbon Radioisotopes, Carcinoma, Ehrlich Tumor, Dihydroxyacetone Phosphate, Lipids, Mice, Microsomes, Radioisotope Dilution Technique, Trioses, Tritium
Abstract

We have previously presented evidence that acyl dihydroxyacetone phosphate is converted to O-alkyl dihydroxyacetone phosphate via an endiol intermediate which then accepts a fatty alcohol to form O-alkyl dihydroxyacetone phosphate. We have further proposed that, in the absence of fatty alcohol, the endiol derivative of acyl dihydroxyacetone phosphate reacts with water to form dihydroxyacetone phosphate. In support of this hypothesis, we have shown, in an O-alkyl generating system, that the amount of hydrogen released from acyl dihydroxyacetone phosphate in the formation of the endiol is greater than the amount of hydrogen lost from the total lipid present at the end of incubation. The discrepancy is greater in the absence of added hexadecanol. The balance of the hydrogen loss can be accounted for by the formation of a non-lipid substance which was identified as dihydroxyacetone phosphate.

Alternate JournalJ. Biol. Chem.
PubMed ID7005213
Grant List1 RO 1 CA 15047-01 / CA / NCI NIH HHS / United States