Department of Anesthesiology

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Dopamine- and cAMP-regulated phosphoprotein (DARPP-32) and dopamine DA1 agonist-sensitive Na+,K+-ATPase in renal tubule cells.

TitleDopamine- and cAMP-regulated phosphoprotein (DARPP-32) and dopamine DA1 agonist-sensitive Na+,K+-ATPase in renal tubule cells.
Publication TypeJournal Article
Year of Publication1989
AuthorsMeister B, Fryckstedt J, Schalling M, Cortés R, Hökfelt T, Aperia A, Hemmings HC, Nairn AC, Ehrlich M, Greengard P
JournalProc Natl Acad Sci U S A
Volume86
Issue20
Pagination8068-72
Date Published1989 Oct
ISSN0027-8424
Keywords2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine, Animals, Antibodies, Monoclonal, Dopamine Agents, Dopamine and cAMP-Regulated Phosphoprotein 32, Fenoldopam, Fluorescent Antibody Technique, Kidney Tubules, Kinetics, Loop of Henle, Macaca fascicularis, Male, Mice, Nerve Tissue Proteins, Nucleic Acid Hybridization, Phosphoproteins, Rabbits, Rats, Rats, Inbred Strains, RNA, Messenger, Sodium-Potassium-Exchanging ATPase
Abstract

The cellular localization of DARPP-32, a dopamine- and cAMP-regulated phosphoprotein of Mr 32,000 that appears to mediate certain actions of dopamine in the mammalian brain by acting as an inhibitor of protein phosphatase 1, was studied in the kidney of several species. DARPP-32 mRNA and DARPP-32-like immunoreactivity were found in the cytoplasm of cells in the thick ascending limb of the loop of Henle. The specific dopamine DA1 agonist SKF 82526 caused a dose-dependent inhibition of Na+,K+-ATPase activity, which could be blocked by SCH 23390, a specific DA1 antagonist, and by PKI-(5-24) amide, a specific inhibitor of cAMP-dependent protein kinase. The results indicate that DA1 dopamine receptors and DARPP-32, an intracellular third messenger for dopamine, are part of the signal-transduction process for dopamine acting on renal tubule cells.

Alternate JournalProc. Natl. Acad. Sci. U.S.A.
PubMed ID2573060
PubMed Central IDPMC298216
Grant ListMH00606 / MH / NIMH NIH HHS / United States
MH40899 / MH / NIMH NIH HHS / United States
MH43230 / MH / NIMH NIH HHS / United States