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Department of Anesthesiology

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DARPP-32 in the ciliary epithelium of the eye: a neurotransmitter-regulated phosphoprotein of brain localizes to secretory cells.

TitleDARPP-32 in the ciliary epithelium of the eye: a neurotransmitter-regulated phosphoprotein of brain localizes to secretory cells.
Publication TypeJournal Article
Year of Publication1986
AuthorsStone RA, Laties AM, Hemmings HC, Ouimet CC, Greengard P
JournalJ Histochem Cytochem
Volume34
Issue11
Pagination1465-8
Date Published1986 Nov
ISSN0022-1554
KeywordsAnimals, Brain Chemistry, Cats, Ciliary Body, Dopamine and cAMP-Regulated Phosphoprotein 32, Epithelium, Fluorescent Antibody Technique, Histocytochemistry, Humans, Macaca mulatta, Nerve Tissue Proteins, Neurotransmitter Agents, Phosphoproteins, Rats, Receptors, Dopamine
Abstract

DARPP-32, a phosphoprotein enriched in dopaminoceptive brain neurons containing the D-1 receptor subtype, probably functions as an intracellular third messenger to mediate some of the physiological effects of dopamine at the D-1 receptor. By immunohistochemistry in rat, cat, Rhesus monkey, and human, we have localized DARPP-32 to the non-pigmented epithelium of the ciliary body, the innermost layer of the bi-layered epithelium responsible for secretion of aqueous humor into the eye. The immunoreactive protein in rat ciliary body, identified by immunolabeling of a ciliary body extract separated by sodium dodecyl sulfate/polyacrylamide gel electrophoresis, is indistinguishable from DARPP-32 derived from rat caudatoputamen. By analogy with brain, we propose that DARPP-32 may act as a third messenger in the ciliary epithelium, probably through a dopaminergic mechanism.

Alternate JournalJ. Histochem. Cytochem.
PubMed ID2877023
Grant ListEY-05454 / EY / NEI NIH HHS / United States
MH-39327 / MH / NIMH NIH HHS / United States