Title | The use of pHluorins for optical measurements of presynaptic activity. |
Publication Type | Journal Article |
Year of Publication | 2000 |
Authors | Sankaranarayanan S, De Angelis D, Rothman JE, Ryan TA |
Journal | Biophys J |
Volume | 79 |
Issue | 4 |
Pagination | 2199-208 |
Date Published | 2000 Oct |
ISSN | 0006-3495 |
Keywords | Action Potentials, Animals, Biophysical Phenomena, Biophysics, Endocytosis, Exocytosis, Green Fluorescent Proteins, Hippocampus, Hydrogen-Ion Concentration, Luminescent Proteins, Models, Neurological, Optics and Photonics, Presynaptic Terminals, Rats, Rats, Sprague-Dawley, Synaptic Transmission |
Abstract | Genetically encoded reporters for optical measurements of presynaptic activity hold significant promise for measurements of neurotransmission within intact or semi-intact neuronal networks. We have characterized pH-sensitive green fluorescent protein-based sensors (pHluorins) of synaptic vesicle cycling at nerve terminals. pHluorins have a pK approximately 7.1, which make them ideal for tracking synaptic vesicle lumen pH upon cycling through the plasma membrane during action potentials. A theoretical analysis of the expected signals using this approach and guidelines for future reporter development are provided. |
DOI | 10.1016/S0006-3495(00)76468-X |
Alternate Journal | Biophys. J. |
PubMed ID | 11023924 |
PubMed Central ID | PMC1301110 |
Grant List | NS24692 / NS / NINDS NIH HHS / United States |