Department of Anesthesiology

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The use of pHluorins for optical measurements of presynaptic activity.

TitleThe use of pHluorins for optical measurements of presynaptic activity.
Publication TypeJournal Article
Year of Publication2000
AuthorsSankaranarayanan S, De Angelis D, Rothman JE, Ryan TA
JournalBiophys J
Volume79
Issue4
Pagination2199-208
Date Published2000 Oct
ISSN0006-3495
KeywordsAction Potentials, Animals, Biophysical Phenomena, Biophysics, Endocytosis, Exocytosis, Green Fluorescent Proteins, Hippocampus, Hydrogen-Ion Concentration, Luminescent Proteins, Models, Neurological, Optics and Photonics, Presynaptic Terminals, Rats, Rats, Sprague-Dawley, Synaptic Transmission
Abstract

Genetically encoded reporters for optical measurements of presynaptic activity hold significant promise for measurements of neurotransmission within intact or semi-intact neuronal networks. We have characterized pH-sensitive green fluorescent protein-based sensors (pHluorins) of synaptic vesicle cycling at nerve terminals. pHluorins have a pK approximately 7.1, which make them ideal for tracking synaptic vesicle lumen pH upon cycling through the plasma membrane during action potentials. A theoretical analysis of the expected signals using this approach and guidelines for future reporter development are provided.

DOI10.1016/S0006-3495(00)76468-X
Alternate JournalBiophys. J.
PubMed ID11023924
PubMed Central IDPMC1301110
Grant ListNS24692 / NS / NINDS NIH HHS / United States