Department of Anesthesiology

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Optical mapping of release properties in synapses.

TitleOptical mapping of release properties in synapses.
Publication TypeJournal Article
Year of Publication2010
AuthorsAriel P, Ryan TA
JournalFront Neural Circuits
Volume4
Date Published2010
ISSN1662-5110
Abstract

Synapses are important functional units that determine how information flows through the brain. Understanding their biophysical properties and the molecules that underpin them is an important goal of cellular neuroscience. Thus, it is of interest to develop protocols that allow easy measurement of synaptic parameters in model systems that permit molecular manipulations. Here, we used a sensitive and high-time resolution optical approach that allowed us to characterize two functional parameters critical to presynaptic efficacy: vesicle fusion probability (Pv) and readily-releasable pool size (RRP). We implemented two different approaches to determine the RRP size that were in broad agreement: depletion of the RRP by high-frequency stimulation and saturation of the calcium sensor during single action potential stimuli. Our methods are based on reporters that provide a robust, quantitative, purely presynaptic readout and present a new avenue to study molecules that affect synaptic vesicle exocytosis.

DOI10.3389/fncir.2010.00018
Alternate JournalFront Neural Circuits
PubMed ID20802854
PubMed Central IDPMC2928663
Grant ListR01 MH085783 / MH / NIMH NIH HHS / United States
R01 MH085783-01A1 / MH / NIMH NIH HHS / United States
R01 MH085783-02 / MH / NIMH NIH HHS / United States