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Nanodissected elastically loaded clathrin lattices relax to increased curvature.

TitleNanodissected elastically loaded clathrin lattices relax to increased curvature.
Publication TypeJournal Article
Year of Publication2021
AuthorsTagiltsev G, Haselwandter CA, Scheuring S
JournalSci Adv
Volume7
Issue33
Date Published2021 Aug
ISSN2375-2548
Abstract

Clathrin-mediated endocytosis (CME) is the major endocytosis pathway for the specific internalization of large compounds, growth factors, and receptors. Formation of internalized vesicles from the flat plasma membrane is accompanied by maturation of cytoplasmic clathrin coats. How clathrin coats mature and the mechanistic role of clathrin coats are still largely unknown. Maturation models proposed clathrin coats to mature at constant radius or constant area, driven by molecular actions or elastic energy. Here, combining high-speed atomic force microscopy (HS-AFM) imaging, HS-AFM nanodissection, and elasticity theory, we show that clathrin lattices deviating from the intrinsic curvature of clathrin form elastically loaded assemblies. Upon nanodissection of the clathrin network, the stored elastic energy in these lattices drives lattice relaxation to accommodate an ideal area-curvature ratio toward the formation of closed clathrin-coated vesicles. Our work supports that the release of elastic energy stored in curvature-frustrated clathrin lattices could play a major role in CME.

DOI10.1126/sciadv.abg9934
Alternate JournalSci Adv
PubMed ID34389539
PubMed Central IDPMC8363152
Grant ListDP1 AT010874 / AT / NCCIH NIH HHS / United States