Department of Anesthesiology

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Molecular crowding on the cell surface.

TitleMolecular crowding on the cell surface.
Publication TypeJournal Article
Year of Publication1988
AuthorsRyan TA, Myers J, Holowka D, Baird B, Webb WW
JournalScience
Volume239
Issue4835
Pagination61-4
Date Published1988 Jan 1
ISSN0036-8075
KeywordsAnimals, Cell Membrane, Membrane Fluidity, Membrane Proteins, Rats, Receptors, Fc, Receptors, IgE, Thermodynamics, Tumor Cells, Cultured
Abstract

Strong steric interactions among proteins on crowded living cell surfaces were revealed by measurements of the equilibrium spatial distributions of proteins in applied potential gradients. The fraction of accessible surface occupied by mobile surface proteins can be accurately represented by including steric exclusion in the statistical thermodynamic analysis of the data. The analyses revealed enhanced, concentration-dependent activity coefficients, implying unanticipated thermodynamic activity even at typical cell surface receptor concentrations.

Alternate JournalScience
PubMed ID2962287
Grant ListAI18306 / AI / NIAID NIH HHS / United States
AI22449 / AI / NIAID NIH HHS / United States
GM33028 / GM / NIGMS NIH HHS / United States