| Title | Molecular crowding on the cell surface. |
| Publication Type | Journal Article |
| Year of Publication | 1988 |
| Authors | Ryan TA, Myers J, Holowka D, Baird B, Webb WW |
| Journal | Science |
| Volume | 239 |
| Issue | 4835 |
| Pagination | 61-4 |
| Date Published | 1988 Jan 1 |
| ISSN | 0036-8075 |
| Keywords | Animals, Cell Membrane, Membrane Fluidity, Membrane Proteins, Rats, Receptors, Fc, Receptors, IgE, Thermodynamics, Tumor Cells, Cultured |
| Abstract | Strong steric interactions among proteins on crowded living cell surfaces were revealed by measurements of the equilibrium spatial distributions of proteins in applied potential gradients. The fraction of accessible surface occupied by mobile surface proteins can be accurately represented by including steric exclusion in the statistical thermodynamic analysis of the data. The analyses revealed enhanced, concentration-dependent activity coefficients, implying unanticipated thermodynamic activity even at typical cell surface receptor concentrations. |
| Alternate Journal | Science |
| PubMed ID | 2962287 |
| Grant List | AI18306 / AI / NIAID NIH HHS / United States AI22449 / AI / NIAID NIH HHS / United States GM33028 / GM / NIGMS NIH HHS / United States |