Department of Anesthesiology

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Imaging [Ca2+]i dynamics during signal transduction.

TitleImaging [Ca2+]i dynamics during signal transduction.
Publication TypeJournal Article
Year of Publication1990
AuthorsRyan TA, Millard PJ, Webb WW
JournalCell Calcium
Volume11
Issue2-3
Pagination145-55
Date Published1990 Feb-Mar
ISSN0143-4160
KeywordsAnimals, Antigens, Antigens, Differentiation, B-Lymphocyte, Benzofurans, Calcium, Fura-2, Image Processing, Computer-Assisted, Immunoglobulin E, Leukemia, Basophilic, Acute, Rats, Receptor Aggregation, Receptors, Fc, Receptors, IgE, Signal Transduction, Tumor Cells, Cultured
Abstract

The elevation of free intracellular Ca2+ activity ([Ca2+]i) is widely recognised as a central event in many signal transduction processes in cellular physiology. Recent advances in optical techniques for measuring [Ca2+]i as well as developments in quantitative low light level fluorescence microscopy have led to the application of these methods to the study of subcellular [Ca2+]i in many biological systems. In the following paper we describe some techniques in our laboratory to provide quantitative high spatio-temporal resolution measurements of [Ca2+]i in individual living cells during the signal transduction of cell surface receptor ligand interactions. In particular, we are studying the changes in [Ca2+]i induced by the micro-aggregation of immunoglobulin E (IgE) receptor complexes on the surface of rat basophilic leukemia (RBL) cells (a tumor mast cell line) by multivalent antigen. We seek to understand the mechanisms which are involved in the detection of these cell surface events which lead to changes in [Ca2+]i as well as the interactions between the various subcellular components which impart the delicate control of [Ca2+]i during cellular stimulation. The limitations and properties of the technology used for these studies will be discussed, and some illustrative examples of the type of [Ca2+]i changes found in this biological system will be given.

Alternate JournalCell Calcium
PubMed ID2141302