GABABR silencing of nerve terminals.

TitleGABABR silencing of nerve terminals.
Publication TypeJournal Article
Year of Publication2023
AuthorsCook DC, Ryan TA
Date Published2023 Apr 04

Control of neurotransmission efficacy is central to theories of how the brain computes and stores information. Presynaptic G-protein coupled receptors (GPCRs) are critical in this problem as they locally influence synaptic strength and can operate on a wide range of time scales. Among the mechanisms by which GPCRs impact neurotransmission is by inhibiting voltage-gated calcium (Ca2+) influx in the active zone. Here, using quantitative analysis of both single bouton Ca2+ influx and exocytosis, we uncovered an unexpected non-linear relationship between the magnitude of action potential driven Ca2+ influx and the concentration of external Ca2+ ([Ca2+]e). We find that this unexpected relationship is leveraged by GPCR signaling when operating at the nominal physiological set point for [Ca2+]e, 1.2 mM, to achieve complete silencing of nerve terminals. These data imply that the information throughput in neural circuits can be readily modulated in an all-or-none fashion at the single synapse level when operating at the physiological set point.

Alternate JournalElife
PubMed ID37014052
PubMed Central IDPMC10115440
Grant ListNS036942 / NS / NINDS NIH HHS / United States
GM148935 / GM / NIGMS NIH HHS / United States
GM148935 / GM / NIGMS NIH HHS / United States
NS117139 / NS / NINDS NIH HHS / United States