Functional reorganization of sensory pathways in the rat spinal dorsal horn following peripheral nerve injury.

TitleFunctional reorganization of sensory pathways in the rat spinal dorsal horn following peripheral nerve injury.
Publication TypeJournal Article
Year of Publication2001
AuthorsOkamoto M, Baba H, Goldstein PA, Higashi H, Shimoji K, Yoshimura M
JournalJ Physiol
Volume532
IssuePt 1
Pagination241-50
Date Published2001 Apr 1
ISSN0022-3751
KeywordsAfferent Pathways, Animals, Electric Stimulation, Excitatory Postsynaptic Potentials, Humans, Male, Nerve Fibers, Pain, Patch-Clamp Techniques, Posterior Horn Cells, Rats, Rats, Sprague-Dawley, Sciatic Neuropathy, Spinal Cord
Abstract

Functional reorganization of sensory pathways in the rat spinal dorsal horn following sciatic nerve transection was examined using spinal cord slices with an attached dorsal root. Slices were obtained from animals whose sciatic nerve had been transected 2-4 weeks previously and compared to sham-operated controls. Whole-cell recordings from substantia gelatinosa neurones in sham-operated rats, to which nociceptive information was preferentially transmitted, revealed that dorsal root stimulation sufficient to activate A afferent fibres evoked a mono- and/or polysynaptic EPSC in 111 of 131 (approximately 85%) neurones. This is in contrast to the response following A fibre stimulation, where monosynaptic EPSCs were observed in 2 of 131 (approximately 2%) neurones and polysynaptic EPSCs were observed in 18 of 131 (approximately 14%) neurones. In sciatic nerve-transected rats, however, a polysynaptic EPSC following stimulation of A afferents was elicited in 30 of 37 (81%) neurones and a monosynaptic EPSC evoked by A afferent stimulation was detected in a subset of neurones (4 of 37, approximately 11%). These observations suggest that, following sciatic nerve transection, large myelinated A afferent fibres establish synaptic contact with interneurones and transmit innocuous information to substantia gelatinosa. This functional reorganization of the sensory circuitry may constitute an underlying mechanism, at least in part, for sensory abnormalities following peripheral nerve injuries.

Alternate JournalJ. Physiol. (Lond.)
PubMed ID11283238
PubMed Central IDPMC2278535