|Title||Dynamin phosphorylation controls optimization of endocytosis for brief action potential bursts.|
|Publication Type||Journal Article|
|Year of Publication||2013|
|Authors||Armbruster M, Messa M, Ferguson SM, De Camilli P, Ryan TA|
Modulation of synaptic vesicle retrieval is considered to be potentially important in steady-state synaptic performance. Here we show that at physiological temperature endocytosis kinetics at hippocampal and cortical nerve terminals show a bi-phasic dependence on electrical activity. Endocytosis accelerates for the first 15-25 APs during bursts of action potential firing, after which it slows with increasing burst length creating an optimum stimulus for this kinetic parameter. We show that activity-dependent acceleration is only prominent at physiological temperature and that the mechanism of this modulation is based on the dephosphorylation of dynamin 1. Nerve terminals in which dynamin 1 and 3 have been replaced with dynamin 1 harboring dephospho- or phospho-mimetic mutations in the proline-rich domain eliminate the acceleration phase by either setting endocytosis at an accelerated state or a decelerated state, respectively. DOI:http://dx.doi.org/10.7554/eLife.00845.001.
|PubMed Central ID||PMC3728620|
|Grant List||P30 DA018343 / DA / NIDA NIH HHS / United States|