We are seeing patients in-person and through Video Visits. Learn more about how we’re keeping you safe and please review our updated visitor policy. Please also consider supporting Weill Cornell Medicine’s efforts to support our front-line workers.
Department of Anesthesiology

You are here

DARPP-32 (dopamine and cAMP-regulated phosphoprotein, M(r) 32,000) is a membrane protein in the bovine parathyroid.

TitleDARPP-32 (dopamine and cAMP-regulated phosphoprotein, M(r) 32,000) is a membrane protein in the bovine parathyroid.
Publication TypeJournal Article
Year of Publication1995
AuthorsMatovcik LM, Hemmings HC, Kinder BK
JournalFEBS Lett
Date Published1995 May 1
KeywordsAnimals, Brain Chemistry, Cattle, Dopamine and cAMP-Regulated Phosphoprotein 32, Fluorescent Antibody Technique, Isoenzymes, Membrane Proteins, Membranes, Microscopy, Immunoelectron, Nerve Tissue Proteins, Parathyroid Glands, Phosphoprotein Phosphatases, Phosphoproteins, Protein Phosphatase 1, Protein Prenylation, Subcellular Fractions, Tissue Distribution

A distinct form of DARPP-32, a protein phosphatase-1 inhibitor, has been identified in bovine calf parathyroid glands. Immunoblot analysis of parathyroid tissue revealed a 32 kDa protein present predominantly in a particulate fraction; it remained particulate after treatment with 1.0 M NaCl or 0.1 M Na2CO3. Metabolic labeling of parathyroid cells with mevalonolactone demonstrated that DARPP-32 is isoprenylated. Immunocytochemical localization studies demonstrated that DARPP-32 is present in vesicles throughout the cytoplasm of parathyroid cells, and that protein phosphatase-1 gamma is concentrated in the region of the plasma membrane. Thus, in contrast to the predominately soluble form of DARPP-32 that has been characterized in selected areas of the central nervous system, the parathyroid form is tightly associated with intracellular membranes.

Alternate JournalFEBS Lett.
PubMed ID7750546