Department of Anesthesiology

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ARPP-21, a cyclic AMP-regulated phosphoprotein enriched in dopamine-innervated brain regions. II. Immunocytochemical localization in rat brain.

TitleARPP-21, a cyclic AMP-regulated phosphoprotein enriched in dopamine-innervated brain regions. II. Immunocytochemical localization in rat brain.
Publication TypeJournal Article
Year of Publication1989
AuthorsOuimet CC, Hemmings HC, Greengard P
JournalJ Neurosci
Volume9
Issue3
Pagination865-75
Date Published1989 Mar
ISSN0270-6474
KeywordsAnimals, Brain, Cyclic AMP, Diencephalon, Dopamine, Immunohistochemistry, Male, Mesencephalon, Phosphoproteins, Rats, Rats, Inbred Strains, Telencephalon, Tissue Distribution
Abstract

ARPP-21, a cAMP-regulated phosphoprotein, has been studied by immunocytochemistry to determine its cellular and regional distribution in rat brain. This study demonstrates that ARPP-21 immunoreactivity is present throughout the cytoplasm of immunoreactive neurons and that most of the immunoreactivity is associated with the basal ganglia. Within the caudatoputamen (CP), nucleus accumbens, olfactory tubercle, bed nucleus of the stria terminalis, and portions of the amygdaloid complex, ARPP-21 is present in neuronal somata and dendrites. In brain regions known to receive projections from these nuclei, immunoreactivity is present in puncta (presumed axons and axon terminals). These regions include the globus pallidus, ventral pallidum, entopeduncular nucleus, lateral preoptic area, and substantia nigra. Within the basal ganglia, ARPP-21 immunoreactivity is most intense in the olfactory tubercle, nucleus accumbens, medial portion of the CP, and the ventral retrochiasmatic pocket of the CP. These same areas comprise the limbic striatum, and ARPP-21 is the first substance found to be specifically enriched therein. The possibility is discussed that ARPP-21 mediates effects of multiple first messengers, including dopamine and vasoactive intestinal polypeptide, that act through cAMP.

Alternate JournalJ. Neurosci.
PubMed ID2538585
Grant ListMH 40899 / MH / NIMH NIH HHS / United States