|Title||Advances in high-speed atomic force microscopy (HS-AFM) reveal dynamics of transmembrane channels and transporters.|
|Publication Type||Journal Article|
|Year of Publication||2019|
|Authors||Heath GR, Scheuring S|
|Journal||Curr Opin Struct Biol|
|Date Published||2019 Mar 14|
Recent advances in high-speed atomic force microscopy (HS-AFM) have made it possible to study the conformational dynamics of single unlabeled transmembrane channels and transporters. Improving environmental control with the integration of a non-disturbing buffer exchange system, which in turn allows the gradual change of conditions during HS-AFM operation, has provided a breakthrough toward the performance of structural titration experiments. Further advancements in temporal resolution with the use of line scanning and height spectroscopy techniques show how high-speed atomic force microscopy can measure millisecond to microsecond dynamics, pushing this method beyond current spatial and temporal limits offered by less direct techniques.
|Alternate Journal||Curr. Opin. Struct. Biol.|