Department of Anesthesiology

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Advances in high-speed atomic force microscopy (HS-AFM) reveal dynamics of transmembrane channels and transporters.

TitleAdvances in high-speed atomic force microscopy (HS-AFM) reveal dynamics of transmembrane channels and transporters.
Publication TypeJournal Article
Year of Publication2019
AuthorsHeath GR, Scheuring S
JournalCurr Opin Struct Biol
Volume57
Pagination93-102
Date Published2019 Mar 14
ISSN1879-033X
Abstract

Recent advances in high-speed atomic force microscopy (HS-AFM) have made it possible to study the conformational dynamics of single unlabeled transmembrane channels and transporters. Improving environmental control with the integration of a non-disturbing buffer exchange system, which in turn allows the gradual change of conditions during HS-AFM operation, has provided a breakthrough toward the performance of structural titration experiments. Further advancements in temporal resolution with the use of line scanning and height spectroscopy techniques show how high-speed atomic force microscopy can measure millisecond to microsecond dynamics, pushing this method beyond current spatial and temporal limits offered by less direct techniques.

DOI10.1016/j.sbi.2019.02.008
Alternate JournalCurr. Opin. Struct. Biol.
PubMed ID30878714