| Title | The use of pHluorins for optical measurements of presynaptic activity. |
| Publication Type | Journal Article |
| Year of Publication | 2000 |
| Authors | Sankaranarayanan S, De Angelis D, Rothman JE, Ryan TA |
| Journal | Biophys J |
| Volume | 79 |
| Issue | 4 |
| Pagination | 2199-208 |
| Date Published | 2000 Oct |
| ISSN | 0006-3495 |
| Keywords | Action Potentials, Animals, Biophysical Phenomena, Biophysics, Endocytosis, Exocytosis, Green Fluorescent Proteins, Hippocampus, Hydrogen-Ion Concentration, Luminescent Proteins, Models, Neurological, Optics and Photonics, Presynaptic Terminals, Rats, Rats, Sprague-Dawley, Synaptic Transmission |
| Abstract | Genetically encoded reporters for optical measurements of presynaptic activity hold significant promise for measurements of neurotransmission within intact or semi-intact neuronal networks. We have characterized pH-sensitive green fluorescent protein-based sensors (pHluorins) of synaptic vesicle cycling at nerve terminals. pHluorins have a pK approximately 7.1, which make them ideal for tracking synaptic vesicle lumen pH upon cycling through the plasma membrane during action potentials. A theoretical analysis of the expected signals using this approach and guidelines for future reporter development are provided. |
| DOI | 10.1016/S0006-3495(00)76468-X |
| Alternate Journal | Biophys. J. |
| PubMed ID | 11023924 |
| PubMed Central ID | PMC1301110 |
| Grant List | NS24692 / NS / NINDS NIH HHS / United States |