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Distribution of protein phosphatase inhibitor-1 in brain and peripheral tissues of various species: comparison with DARPP-32.

TitleDistribution of protein phosphatase inhibitor-1 in brain and peripheral tissues of various species: comparison with DARPP-32.
Publication TypeJournal Article
Year of Publication1992
AuthorsHemmings HC, Girault JA, Nairn AC, Bertuzzi G, Greengard P
JournalJ Neurochem
Volume59
Issue3
Pagination1053-61
Date Published1992 Sep
ISSN0022-3042
KeywordsAnimals, Brain, Carrier Proteins, Central Nervous System, Dopamine and cAMP-Regulated Phosphoprotein 32, Enzyme Inhibitors, Female, Intracellular Signaling Peptides and Proteins, Male, Nerve Tissue Proteins, Phosphoproteins, Proteins, Rats, Rats, Inbred Strains, Subcellular Fractions, Tissue Distribution, Tyrosine 3-Monooxygenase, Vertebrates
Abstract

The distribution of inhibitor-1, a cyclic AMP-regulated inhibitor of protein phosphatase-1, was analyzed in various brain regions and peripheral tissues of various species by immunolabeling of sodium dodecyl sulfate-poly-acrylamide gel transfers using specific antibodies. The distribution of inhibitor-1 was directly compared to that of DARPP-32, a structurally related cyclic AMP-regulated inhibitor of protein phosphatase-1. In rat CNS, a single immunoreactive protein of M(r) 30,000, identified as inhibitor-1, was widely distributed. In contrast, DARPP-32 was highly concentrated in the basal ganglia. Inhibitor-1 was detected in brain tissue from frog (M(r) 27,000), turtle (M(r) 29,000/33,000), canary (M(r) 26,000), pigeon (M(r) 28,000), mouse (M(r) 30,500), rabbit (M(r) 26,500), cow (M(r) 27,000), and monkey (M(r) 27,500), but not from goldfish. Inhibitor-1 was detected at various levels in most peripheral tissues of the species studied; however, it was not detectable in certain tissues of particular species (e.g., rat and cow liver). DARPP-32 was detected in brain tissue of all the species tested except frog and goldfish, but was not detectable in most peripheral tissues. Both inhibitor-1 and DARPP-32 were concentrated in the cytosol and synaptosomal cytosol of rat striatum. The developmental expressions of inhibitor-1 and DARPP-32 in rat striatum differed: the level of inhibitor-1 peaked in the first postnatal week and then declined by the third postnatal week, whereas the level of DARPP-32 increased to a peak level by the third postnatal week and remained elevated thereafter.(ABSTRACT TRUNCATED AT 250 WORDS)

Alternate JournalJ. Neurochem.
PubMed ID1353788
Grant ListMH-40899 / MH / NIMH NIH HHS / United States