Title | Septin higher-order structure on yeast membranes in vitro. |
Publication Type | Journal Article |
Year of Publication | 2025 |
Authors | Goodchild JA, Curtis BN, Pan Y, Jiang Y, Jiao F, Gladfelter AS, Scheuring S |
Journal | Nat Commun |
Volume | 16 |
Issue | 1 |
Pagination | 5055 |
Date Published | 2025 May 30 |
ISSN | 2041-1723 |
Keywords | Cell Membrane, Cytoskeleton, Lipid Bilayers, Microscopy, Atomic Force, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Septins |
Abstract | Septins are cytoskeletal proteins that form filaments and higher-order structures, and remodel membranes in a variety of processes. Structural and cell biological studies provided atomic- and micro-scale details, but the understanding of septin assembly at the mesoscale is limited. Here, we used high-speed atomic force microscopy (HS-AFM) to analyze yeast septin assembly on yeast supported lipid bilayers (SLBs). We found the coexistence of three lipid phases in yeast membranes, where septin polymerized selectively on the liquid-disordered phase. Septin filaments adhered to membranes with a conserved face; and paired filaments, previously reported in less native environments, were not observed. Additionally, septin filaments exhibited lateral and longitudinal alignment. We used HS-AFM force-sweep experiments to disrupt septin structures and observe organizational recovery through self-templating. Finally, septin filaments stacked, where higher layer filament alignment was templated by the layer below. Thus, septins encode their 3D-structural organization, likely tunable by the membrane and bulk environment. |
DOI | 10.1038/s41467-025-60344-w |
Alternate Journal | Nat Commun |
PubMed ID | 40447632 |
PubMed Central ID | PMC12125352 |
Grant List | R01GM130934 / / U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) / DP1AT010874 / / U.S. Department of Health & Human Services | NIH | National Center for Complementary and Integrative Health (NCCIH) / R01 NS134559 / NS / NINDS NIH HHS / United States R01NS134559 / / U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke (NINDS) / DP1 AT010874 / AT / NCCIH NIH HHS / United States R01 GM130934 / GM / NIGMS NIH HHS / United States |