|Phosphorylation of CREB and DARPP-32 during late LTP at hippocampal to prefrontal cortex synapses in vivo.
|Year of Publication
|Hotte M, Thuault S, Dineley KT, Hemmings HC, Nairn AC, Jay TM
|Animals, Cyclic AMP Response Element-Binding Protein, Dopamine and cAMP-Regulated Phosphoprotein 32, Hippocampus, Long-Term Potentiation, Male, Phosphorylation, Prefrontal Cortex, Rats, Rats, Sprague-Dawley, Synapses
Specific patterns of stimulation applied in the ventral hippocampus produce long-term potentiation (LTP) of postsynaptic synapses in the prefrontal cortex in vivo. The induction of LTP is dependent on NMDA receptors and cAMP-dependant kinase (PKA) activation. Yet little is known concerning the cellular mechanisms underlying the expression of this neocortical form of LTP. In the present study, we tested whether LTP at hippocampal to prefrontal cortex synapses leads to activation of DARPP-32 and CREB as well as defined the temporal regulation of the phosphorylation states of both proteins. Our data indicate a peak in CREB and DARPP-32 phosphorylation during the late phase of prefrontal LTP (2 h posttetanus). These findings support the hypothesis that prolonged expression of hippocampal-prefrontal cortex LTP depends on a synergistic mechanism involving phosphorylation of both CREB and DARPP-32 via activation of the cAMP/PKA-dependent pathway.
|DA 10044 / DA / NIDA NIH HHS / United States
P01 DA010044 / DA / NIDA NIH HHS / United States
R01 GM 58055 / GM / NIGMS NIH HHS / United States
R01 GM058055 / GM / NIGMS NIH HHS / United States