Department of Anesthesiology

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Functional Connectivity under Optogenetic Control Allows Modeling of Human Neuromuscular Disease.

TitleFunctional Connectivity under Optogenetic Control Allows Modeling of Human Neuromuscular Disease.
Publication TypeJournal Article
Year of Publication2016
AuthorsSteinbeck JA, Jaiswal MK, Calder EL, Kishinevsky S, Weishaupt A, Toyka KV, Goldstein PA, Studer L
JournalCell Stem Cell
Volume18
Issue1
Pagination134-43
Date Published2016 Jan 7
ISSN1875-9777
Abstract

Capturing the full potential of human pluripotent stem cell (PSC)-derived neurons in disease modeling and regenerative medicine requires analysis in complex functional systems. Here we establish optogenetic control in human PSC-derived spinal motorneurons and show that co-culture of these cells with human myoblast-derived skeletal muscle builds a functional all-human neuromuscular junction that can be triggered to twitch upon light stimulation. To model neuromuscular disease we incubated these co-cultures with IgG from myasthenia gravis patients and active complement. Myasthenia gravis is an autoimmune disorder that selectively targets neuromuscular junctions. We saw a reversible reduction in the amplitude of muscle contractions, representing a surrogate marker for the characteristic loss of muscle strength seen in this disease. The ability to recapitulate key aspects of disease pathology and its symptomatic treatment suggests that this neuromuscular junction assay has significant potential for modeling of neuromuscular disease and regeneration.

DOI10.1016/j.stem.2015.10.002
Alternate JournalCell Stem Cell
PubMed ID26549107
PubMed Central IDPMC4707991
Grant ListP30 CA008748 / CA / NCI NIH HHS / United States
R01 NS052671 / NS / NINDS NIH HHS / United States